FAQs

Any method that produces highly pure DNA greater than 10 ng/ul is acceptable.

HemeScreen and Bloodhound assays require a minimum 7 feet of bench space; 1 foot for instrumentation and 6 feet of working bench space.

QuantStudio3, QuantStudio5, QuantStudio6, QuantStudio7, 7500 or any other high quality qPCR system with a 96-well block that can fit FAST PCR plates and has ramp rate = or greater than 1.6 °C/sec. For more detailed info, click here.

The total process time to run the assay from DNA extraction to data generation is 3-4 hours depending upon the number of samples run.

Data is saved to a secure cloud storage and is also saved locally on the instrument for backup.

IV-cell is an all-in-one media that requires no cocktailing or mixing of mitogens when preparing a cell culture. Through its proprietary chemistry, the media enables the lab to culture all four cell lineages simultaneously.

The cell culturing process requires the selection of the cell lineage to be cultured (B-cell, T-Cell, Myeloid or plasma). This is typically done when the ordering physician provides a clinical suspicion of the possible malignancy. However, data shows that in approximately 40% of cases, the clinical suspicion is incorrect, which results in the culturing of the wrong cell lineage. IV-Cell enables the laboratory to utilize information from flow cytometry and morphologic assessment to ensure that the correct cell lineage is being cultured.

IV-Cell has demonstrated superior quality in identifying abnormalities in all cytogenetic-related hematologic malignancies. It is easier to use because it is a complete product and requires no cocktailing with other components or mitogens. IV-Cell also produces 400+ banding resolution, enabling rapid and accurate chromosome analysis.

This is managed via the culture time. Mimicking the human body, the cell lineages “grow” at a different pace. therefore, the culture time (the time the sample is left in the media) will determine the desired cell lineage to be cultured. Once the culturing time has elapsed, harvesting the culture will yield the correct cell lineage.

This depends on the cell lineage of interest. Myeloid cells require culturing of 48 hours, whereas T-cells require 72 hours.

The media does not require freezing and is stored at refrigerator temperature (2-6C). This makes it easy to use because the lab technician does not need to take into account freeze/thaw cycles.

No, the current media is only for adult hematologic malignancies.

Typically laboratories use between 5-10ml per culture, depending on the sample concentration (assuming a cell count of bbetween 0-10 x106 cells/ml).

No. Because of the unique structure of the media, the cells can be cultured directly in the tube, avoiding the need for transfer to the flask. It also does not require any CO2 during incubation.

IV-Cell is intended for use with bone marrow or peripheral blood samples.