CaseVuBloodhound Quantitative BCR-ABL1 Panel
At Precipio’s clinical lab, our Bloodhound quantitative BCR-ABL1 molecular panel goes beyond standard breakpoint testing. It provides comprehensive results in a simple workflow. The assay’s 0.001% limit of detection (LoD) offers superior MRD monitoring for four breakpoints (p.190, p.203, p.210, & p.230).
Bloodhound BCR-ABL1 panel has been validated as a lab developed test at Precipio. It has not been approved by the FDA.
Tech Overview BCR-ABL1 SDS
Development Of Bloodhound BCR-ABL1 Panel
Precipio’s clinical hematopathology laboratory developed the Bloodhound BCR-ABL1 molecular panel to enhance turn-around time and provide more accurate, comprehensive BCR-ABL molecular testing. By adopting a panel approach to gene mutation detection and MRD monitoring, Precipio’s clinical lab efficiently identifies single mutations, mutation changes, and co-expression across four BCR-ABL1 breakpoints (p.190, p.203, p.210, and p.230) in a single assay on one RT-PCR platform. As a result, our lab achieves a more efficient testing workflow, which in turn enables rapid turn-around times. All this while providing more molecular information to clinicians.
BCR-ABL1 Molecular Panel Configurations
The BCR-ABL1 panel comes pre-plated or as reagent sets with optimized primers and embedded mutant, NTC, and wild type controls for p.190, p.203, p.210, and p.230 breakpoints. This simplifies lab inventory management by providing a complete molecular assay without tracking multiple SKUs of single gene testing assays that require separate control kits. This also means fewer lot to lot QC checks are required compared to individual assays for each breakpoint that is tested.
- 4-Sample pre-plated plate
- 8-Sample pre-plated plate
- Vialed free flow reagent set
BCR-ABL1 Assay Workflow
The process begins with standard RNA isolation and cDNA preparation techniques to prepare the sample. Next, data is captured on the RT-PCR instrument and uploaded for automated analysis, which determines the presence and relative ratio of the p.190, p.203, p.210, and p.230 breakpoints.