BloodhoundTM MPN Panel
Blood Cancer Molecular Testing
Bloodhound Quantitative MPN Panel
The MPN panel provides comprehensive results in a simple workflow with 0.5% limit of detection for JAK2 V617F mutations and 2% limit of detection for other JAK2, MPL and CALR targets. Pre-plated configurations streamline molecular testing and command higher reimbursements for Precipio’s clinical lab.
At Precipio’s clinical lab, our Bloodhound Quantitative MPN panel is used to detect somatic mutations associated with various myeloproliferative neoplasms including Myelofibrosis, Polycythemia Vera (PV) and Essential Thrombocythemia (ET). The molecular panel quantifies allele frequencies of targeted mutations in JAK2 V617F, JAK2 Exon 12, JAK2 Exon 13, MPL, CALR type 1 and CALR type 2. Bloodhound MPN molecular tests have been validated as lab developed tests at Precipio. They have not been approved by the FDA.
Development Of The Bloodhound Quantitative MPN Panel
Precipio’s own clinical hematopathology laboratory developed the Bloodhound MPN panel to address the issues of turn-around time and comprehensive molecular testing. We take a panel approach to gene mutation detection to identify incidence of single mutations, mutation changes, and co-expression in a single assay run on the same platform as our complementary Bloodhound BCR::ABL1 and HemeScreen assays. This provides our lab with a more efficient testing workflow and also enables rapid turn around time. The MPN Panel detects driver mutations associated with Myeloproliferative Neoplasms including JAK2, CALR, and MPL mutations. This portfolio of assays supports Precipio’s clinical lab to detect and triage MPN molecular status.
MPN Molecular Panel Configurations
The MPN panel comes pre-plated or as reagent sets with optimized primers and embedded mutant, NTC, and wild type controls for detection JAK2 Exon 14 (v617f), JAK Exon 12, JAK2 Exon 13, MPL, CALR type 1, and CALR type 2.
4 and 6-sample pre-plated, and vialed free flow configurations are available for the MPN panel. Custom configurations are also possible.
MPN Assay Workflow
Sample preparation is based on standard DNA extraction techniques. The Bloodhound MPN assay also runs on most HRM-enabled RT-PCR instruments. Automated analysis identifies quantitative status of JAK2 V617F, JAK2 Exon 12, JAK2 Exon 13, MPL, CALR type, and CALR type 2 gene mutations.
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