CaseVuBloodhoundTM Panels
Bloodhound Molecular Panels for BCR-ABL1 and MPN
At Precipio’s clinical lab, our Bloodhound BCR-ABL1 molecular panel is used to go beyond standard breakpoint testing. It provides comprehensive results in a simple workflow with 0.001% sensitivity for superior MRD monitoring. Complementary ABL1 mutation resistance and MPN driver mutation panels streamline case work-ups while commanding higher reimbursements for Precipio’s clinical lab. Bloodhound BCR-ABL1, ABL1 Resistance, and MPN molecular tests have been validated as lab developed tests at Precipio. They have not been approved by the FDA.
Bloodhound Brochure Tech Overview BCR-ABL1 Tech Overview ABL1 Resistance Tech Overview MPN
Development Of Bloodhound BCR-ABL1, ABL1 Resistance and MPN Panels
Precipio’s own clinical hematopathology laboratory developed the Bloodhound molecular panels for BCR-ABL1 and MPN to address the issues of turn-around time and accurate, comprehensive molecular testing. Precipio’s clinical hematopathology lab takes a panel approach to gene mutation detection and MRD monitoring of BCR-ABL1 breakpoints to identify incidence of single mutations, mutation changes, and co-expression in a single assay run on one platform for four breakpoints (p.190, p.203, p.210, and p.230). This provides our lab with a more efficient testing workflow and also enables rapid turn around time.
In addition to breakpoint detection and monitoring, Precipio also has developed complementary assays to detect ABL1 gene mutation clusters associated with specific therapeutic indications, enabling the lab to eliminate the need for reflex sequencing of the ABL1 gene, where otherwise necessary.
Precipio also leverages their Bloodhound MPN Panel which detects driver mutations associated with Myeloproliferative Neoplasms including JAK2, CALR, and MPL mutations. This portfolio of assays supports Precipio’s clinical lab to detect, monitor and triage BCR-ABL1 and MPN molecular status.
Additional Bloodhound Molecular Panel Details
BCR-ABL1 Panel
BCR-ABL1 Molecular Panel Configurations
The BCR-ABL1 panel comes pre-plated or as reagent sets with optimized primers and embedded mutant, NTC, and wild type controls for p.190, p.203, p.210, and p.230 breakpoints. This simplifies lab inventory management by providing a complete molecular assay without tracking multiple SKUs of single gene testing assays that require separate control kits.
4, 8 and 12-sample pre-plated, and vialed free flow configurations are available for the quantitative BCR/ABL1 panel. Custom configurations are also possible.
BCR-ABL1 Assay Workflow
Sample preparation is based on standard RNA isolation and cDNA preparation techniques. Most HRM-enabled RT-PCR instruments can run the BCR-ABL1 assay after cDNA samples are plated. In real time, the instrument captures and uploads data for automated analysis to determine the presence and relative ratio of p.190, p.203, p.210, p.230 breakpoints.
ABL1 Resistance Panel
ABL1 Resistance Molecular Panel Configurations
The ABL1 Resistance panel comes pre-plated or as reagent sets with optimized primers and embedded mutant, NTC, and wild type controls for ABL1 Exons 4, 5, 6, and 7.
3 and 6-sample pre-plated, and vialed free flow configurations are available for the ABL1 Resistance panel. Custom configurations are also possible.
ABL1 Resistance Assay Workflow
Sample preparation is based on standard RNA isolation and cDNA preparation techniques. ABL1 resistance testing can leverage sample preparations from the Bloodhound BCR-ABL1 assay. ABL1 Resistance assay also runs on most HRM-enables RT-PCR instruments. In real time, the instrument captures and uploads data for melt curve analysis of ABL1 exons 4, 5, 6, and 7.
MPN Panel
MPN Assay Workflow
MPN Molecular Panel Configurations
The MPN panel comes pre-plated or as reagent sets with optimized primers and embedded mutant, NTC, and wild type controls for detection JAK2 Exon 14 (v617f), JAK Exon 12, JAK2 Exon 13, CALR, and MPL.
4 and 8-sample pre-plated, and vialed free flow configurations are available for the MPN panel. Custom configurations are also possible.
MPN Assay Workflow
Sample preparation is based on standard DNA extraction techniques. The Bloodhound MPN assay also runs on most HRM-enabled RT-PCR instruments. Automated analysis identifies quantitative mutation status of JAK2, CALR, and MPL genes.